1/6/2023 0 Comments Geneious r11 crack![]() ![]() ![]() KO-spanning PCR (S) allowed to identify candidates with predicted biallelic (B) cassette integration showing a single larger band, whereas clones with both targeted size and wild type (WT) size fragments are predicted to be targeted monoallelically (M). Nearly all colonies showed correct KO-flanking PCR (F) band indicating KO cassette integration in at least one allele. ![]() B, representative genotyping PCRs of G418-resistant colonies. Genotyping primers for KO-spanning PCR (S) and KO-flanking PCR (F) are represented with arrows. For constitutive gene knockout the KO cassette is targeted to the first exon of the GOI, or to the first exon shared by all known GOI isoforms (LPGAT1). A, targeting scheme for two genes of interest (GOI): ZDHHC5 and LPGAT1. Gene targeting with selectable KO cassette and genotyping of resistant clones. ![]()
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